Aim: Establish the available scientific evidence of the operational characteristics of PCR-RFLP test with Agar Dilution for the determination of antimicrobial susceptibility in clarithromycin clinical isolates of Helicobacter pylori. Methods: We have performed the search bibliography about diagnostic tests on antimicrobial resistance of H. pylori to clarithromycin by using Agar Dilution and PCR-RFLP techniques over Medline, Science direct, Ovid and Cochrane of studies. The information was validated by two observers who checked the inclusion criteria and quality. We obtained the operational characteristics of the studies on contingency tables; analysis was performed on the RevMan 5 program. Results: A total of 50 references were tested from those 12 has been chosen in accord with the inclusion criteria and analyzed as summary measures. The specificity was a 100 % (CI 95% 91-100) that demonstrate a low probability of positive false. The projected overall sensitivity was 91% (CI 95% 88-94%) which indicated a high probability of negative results. The test showed heterogeneity studies homogeneous in sensitivity and specificity (p = 0.78) (p = 0.99). The graphics of Funnel Plot revealed asymmetry for both of those characteristics that showed a publications bias. In the group analysis have not found antibiotics different from clarithromycin and was evident that the continent was more publications Europe, followed by Asia and Latin America. Conclusion: The sensitivity and specificity of PCR-RFLP technique for clarithromycin not have values equal to or higher than 95% compared proof Agar Dilution.