In the project “RFID from Farm to Fork” an implementation of RFID technologies will be used along the food supply chain: from farm to the consumer. The paper is intended to highlight two examples of how to define farmed fish traceability system suitable for small and medium sized enterprises (SMEs). The first one presents a change from a manual collection of data to an electronic RFID implementation in a small company that performs a complete supply chain from fish farm to retail and private customers. In the second one, a part of already automated process of packing fish using barcode labeling will be upgraded by RFID technology, and traceability will be extended back to breeding and on-growing fish farms, which currently use manual collection of data. The proposed design and selection of fixed and mobile RFID readers in different steps of pilot implementation are defined as modules which could be used as a general approach to apply an automated business process.