Objective: To determine the seroprevalence of HTLV screening and factors associated with co-infection in blood donors in Colombia. Materials and Methods: We performed a retrospective cross-sectional study inferential, the population was of 971 records of volunteer blood donors, we calculated prevalence of HTLV and calculated odds ratios (OR). Results: 49.3 % of donors were between 18 and 33 years, 53.4 % were female, 44.3 % lived with a partner, it was observed that the major syphilis coinfections are 7.0 %. There is a trend to increased prevalence of HTLV between 2010 (0.23 %) and 2011 (0.24 %) in the process of Bogota. Headquarters in related factors associated, positive association was found with increasing age and living with partner, and negative association with con-tributory system in multiple Poisson regression showed that the prevalence of co-infection is 2.92 (95 % CI 1.92 to 4.45) times in people 34 to 64 years compared to under 34 years, also the contributory scheme protects coinfection 0.69 (95 % CI 0.48-0.99) in people with contributory scheme compared to the subsidized regime. Conclusion: In general there is a significant proportion of donors with reactive results are set out clearly and factors associated with coinfection prevalence is higher than in other reports.