The aim of this systematic review and meta-analysis is to determine the efficacy of eradicating Helicobacter pylori for prevention of gastric cancer. We conducted a literature review using major databases including PUBMED, EMBASE, CINAHL (EBSCO), Google Scholar, LILACS, Cochrane, ProQuest Dissertations and Theses. Seven experiments were selected out of the 3,934 references obtained by applying our inclusion and exclusion criteria. All seven were randomi- zed controlled trials. The quality of the studies was assessed with the Cochrane assessment of risk of bias. Statistical analysis was performed with REVMAN 5.2. Out of a total of 5,552 subjects, 55 (2.41%) of the 2,278 patients who had had H. pylori eradicated develo- ped gastric cancer, but 96 (4.22%) of the 2,272 patients who had not had the bacteria eradicated developed gastric cancer (RR: 0.57, CI = 0.42 to 0.79). Follow-up time ranged from 3 to 15 years. The analysis of heterogeneity (Chi square) had a non-significant p value (p = 0.48) confirming the absence of heterogeneity and allowing the meta-analysis. Funnel Plot analysis was used to discard publication bias, and the sensitivity analysis showed no significant changes. In conclusion, this study suggests that eradication of H. pylori reduces the risk of gastric cancer, particularly in high-risk populations with medium quality evidence. We recommend the practice of using eradicate of H. pylori as a preventive measure.