The aims of this work is to develop and validate an alternative analytic method by spectrofluorometry for the quality control of Furosemide in solid pharmaceutical forms based on the native fluorescence of the molecule in acidic medium (?Exc.= 280nm and ?Emi.= 410nm). This method allows to determine Furosemide in a buffer solution of potassium chloride-hydrochloric acid 50 mM (pH 2.0) in a range of 1.45x10-7 to 1.51x10-5 mol L-1 (4.5 to 5000 ng mL-1), with an estimated detection limit of 4.84x10-8 -mol L-1 (1.5 ng mL-1). The proposed method offers, as competitive advantages comparing to the official method reported in the United States Pharmacopeia (USP 29), its simplicity, low cost and frequency of analysis, around 20 samples per hour.