The role of mechanical stimulation for mesenchymal stem cells (MSCs) differentiation is a therapeutic alternative for applications in tissue engineering. The aim of this study was to evaluate the effect of mechanical strain on the differentiation and cellular mechanisms of mechanotransduction in MSCs. The cells were seeded in 75cm2 culture flasks and then exposed to uniaxial mechanical tensile strain of 500, 1000, 1500 and 2000 micro strains (μ ), 9 cycles / minute during 3 hours for 4 consecutive days. Runx2 and Sox9 transcription factors andOsteocalcin (OC), Collagen Type 1 (Col-1) and Alkaline Phosphatase (ALP) gene expression was ascertained. After exposure to mechanical strain, osteogenic marker genes Col-1, OC, and ALP were expressed temporally, while Runx2 and Sox9 transcription factors expression decreased, compared with control cells without stimulation, suggesting that mechani* Correspondencia: Jorge Arturo Rey Cubillos, DDS MSc. Carrera 11 B No 135 A – 08. Interior 5 Apto 201. Bogota, D.C. Tel. 571 – 6147298 3108729347 Email: reyjorgearturo@gmail.com