A new species of Columnea belonging to section Ortholoma (Gesneriaceae) from Antioquia Department in Colombia (Cordillera Occidental) is described and illustrated. This species is the second one known in Columnea to have a corolla with 4 external appendages; the first one described with this trait was C. paraguensis. This trait adds to the knowledge on the diversity of corolla architecture in Columnea, and points out an effect of pollinators on the diversification process in this plant lineage.