In recent years there have been an increasing number of studies on carrion flies due to the medical importance and development of forensic entomology. Knowledge of the species involved in a particular space and time is essential to apply forensic techniques. An updated checklist of carrion flies (Diptera, Calyptratae) from the Valle de Aburra, Antioquia, Colombia is presented as a result of one year sampling using Van Someren Rydon traps. Ninety four species were identified (14 Calliphoridae, 12 Fanniidae, 20 Muscidae, 48 Sarcophagidae), fourteen of these are new records from Colombia, twenty five are new records for the province, one Fannid is a new species, and the first neotropical record of Ravinia querula is reported; finally species diversity of the community and faunistics aspects of some species are discussed.