Application of a methodology to determine acute toxicity and toxic charge of the six effluents of Ecopetrol's production field La Cira-lnfantas, as well as to assess water quality of receptor streams (La Cira and Reposo), through toxicity test with three native organisms from the region: an algae (Scenedesmus subspicatus), a macrophite (Lemna aequinoctialis) a cladoceran (Moinodaphnia macleayi) and Daphnia pu/ex as the reference organism. The toxic potential of the effluents, by conducting additional bioassays with (BaC+2 y NaCI), was also determined since high concentrations of (Ba+2 and Cl-;) are present in the effluents. All tested organisms showed sensitivity to the reference toxicant (K2Cr2O7), and met international criteria fortheir use as bioindicators in ecotoxicology studies. The response of the bioindicators to the effluents depended on each organism; however effluents 2 and 3, the more highly contaminated, presented the highest toxicities.