Two sampling activities (March 2007 and April 2008) were carried out in the municipal rural settlement of Morales (Caloto-Cauca) in order to evaluate the day-butterfly diversity associated with four intervened biotopes: riparian forest, sugarcane plantations, coffee-plantain crop and natural fence. The 1594 individuals were collected using Van Someren-Rydon traps, belonging to 90 species from six families; Nymphalidae with 60 species from the subfamilies Nymphalinae (14), Charaxinae (6), Morphinae (2), Satyrinae (5), Heliconiinae (8), Ithomiinae (6), Danainae (3), Acraeinae (5), Limenitidinae (2), Biblidinae (5) and Melitaeinae (4), Pieridae (11) Hesperiidae (6), Papilionidae (7), Lycaenidae (4) and Riodinidae (2). Additionally, 39% of the species were polyphagous and generalists, represented by the genera Anartia (144 individuals), Actinote (69), Caligo (14), Urbanus (72), Anteos (44), Phoebis (56) and Eurema (223). All these have been recognized as genera associated to anthropogenic intervened areas. The presence of the Satyrinae subfamily (108 individuals) and the Morpho genus (9 individuals) in the study * Recibido 18 de abril de 2009, aceptado 10 de julio de 2009. 1 Departamento de Biologia. Universidad del Valle, Calle 13 # 100 00 Sede Melendez. Cali – Colombia. A.A 25360, Cali. E-mail: lepidoptera.azul@gmail.com 2 Departamento de Biologia. Universidad del Valle, Calle 13 # 100 00 Sede Melendez. Cali – Colombia. A.A 25360, Cali. E-mail: pachacon@univalle.edu.co 3 Departamento de Biologia. Universidad del Valle, Calle 13 # 100 00 Sede Melendez. Cali – Colombia. A.A 25360, Cali. E-mail: ecologia@univalle.edu.co ISSN 0123 3068 bol.cient.mus.hist.nat. 13 (1): 185 195