Colombia is the country with the largest richness in species of glass frogs, but there are gaps in the knowledge of many species distribution. In this article for the first time we report the first records of Centrolene quindianum, Centrolene savagei, Nymphargus grandisonae and Nymphargus spilotus in the municipality of Manizales, Caldas, Colombia, based on specimens collected in the northeastern region of this municipality, on the western flank of the Colombian Cordillera Central. The report of C. quindianum represents the first record for the department of Caldas, filling a distributional hiatus between its nearest locations.