From tissue culture activelygrowing axillary buds of blackberry Rubus glaucus L. has been achieved suitable plants to initiate a micropropagation system. This is the most appropiate method for produce plants in a short period than another method. The explants were grown on Murashige and Skoog (MS) medium supplemented with inositol (100 mg/l), thiamine-HCI (0.4 mg/l) sucrose (30 g/l) and growth regulators. The pH was adjusted to 5.7-5.8 prior to autoclaving. The glass tubes with the medium were sealed and autoclaved for 20 min at 120°C and 20 P.S.I. The explants were desinfected in 0.5% sodium hypoclorite for 5 min and aseptically transferred to test tubes and sealed. The cultures were maintained at day/night temperature of 24°C/28°C under 18 hr of light. Both liquid-paper and agar solidified media were evaluated; the liquid-paper had more advantages. Ascorbic acid (100 mg/l) was added to liquid-paper medium in order to control oxidation. About 8 weeks later, better results on survival and growth were obtained by the addition of benzylaminopurine-BAP (2 ppm), indolacetic acid-AIA (0.1 ppm) and gibberellic acid (1 ppm) to MS medium.