On 335 pollen samples collected from proboscis and eyes of butterflies from the headquarters of the Rio Roble atthe departament of Quindio (Colombia), 31 pollen grains were identified. The most visited plants by butterflies belonging to species of Psychotria (Rubiaceae), Tournefortia (Boraginaceae), Gurania (Cucurbitaceae), Dicliptera (Acanthaceae), and Erato vulcanica (Asteraceae). According to the relation between plants and butterflies two groups were differentiated: generalist as Heliconius clysonimus, Dione juno, Elzunia humboldtll and specialist as Dismorphiacrissia and Phoebis rurina. Butterflies with the higher numbers of plants visited were Heliconius clysonimus, Oleria cadcana, and Godyris quinta.