The biodegradation of diesel in water was done by means of the microorganisms present in an agriculture soil. The kinetics of biodegradation and adsortion of diesel were determined in order to aplying the procedure in soil and water resources contamined with diesel. The mehodology and results of biodegradation and adsortion of diesel in sintetic water is presented with a soil characterizacion. Degradation take place using the original microorganisms present in the soil but giving nitrogen as nutrient. As as oxigen source the hidrogen peroxid was used. The kinetics of diesel volatility are presented too. Kinetics equations for degradation, adsortion and speed constant were determined with the obtained results.