The clarification of cane juice is made by the addition of vegetable mucilage. The objective was to evaluate the clarification of three natural flocculants (balso, cadillo and guasimo) in cane juice. The physicochemical properties and insoluble solids retention were characterized versus a synthetic flocculant. Was used a statistical model for randomized block and control sample was the Profloc 985. Mucilage was obtained by pulping the highest quality distilled water, dried at 38°C for 11 hours and reduced to a particle size of 212µm. The ideal clarification was at a concentration 0,03% w/v. The cadillo was dry flocculant showed greater efficiency in the precipitation of soluble solids to 93,6%, followed by the balso with a 90,4% and 89,7% guacimo, compared to 100% of the control sample. The three modified flocculants are composed mainly of carbohydrates (fructose, glucose and maltose), saponins and phenols, and iron, calcium and phosphate. By comparing the contents of calcium and phosphates was found that higher values correspond to the treatments modified cadillo and balso, these values influenced their best performance in the process of clarification of sugar cane juice.