SUMMARY Pre-Hispanic cultures that lived in territories of Central and South America practiced meliponiculture, extracting and processing honey and other products from several stingless bee species. Colonization of these territories introduced beekeeping of Apis mellifera, and practically eliminated meliponiculture. Nevertheless, honey of the species Trigona angustula (Latreille, 1811), called ‘miel de angelita’ (or ‘little-angel honey’) has retained a place in the traditional market, with about ten times the cost of A. mellifera honey. Due to its scarcity and to the fact that it is popularly considered to have medicinal properties. Although there are noticeable differences between miel de angelita and common A. mellifera honeys, the lack of objective quality standards has often allowed falsification. Thus, assessment of its physicochemi cal properties is of interest. In this study, the main nutritional and physicochemical characteristics were analyzed in 44 samples from seven regions of Colombia. Results showed a major composition of 24.3 ±2.3% moisture, 23.5 ±6.4% glucose, 30.1 ±5.4% fructose and 4.2 ±2.4% disaccharides (sucrose and maltose). The honeys presented 0.205 ±0.070% ash, 155.0 ±65.1ppm Na, 576.6 ±177.6ppm K, 199.6 ±63.4ppm Ca, 56.0 ±27.5ppm Mg, 5.8 ±2.3ppm Fe, 0.9 ±0.3ppm Cu, and 19.6 ±8.3 Zn. Free acidity, hydroxymethylfurfural (HMF) content, pH, conductivity, specific ro tation, diastase activity and color (on the Pfund scale) were also assessed. This study constitutes the basis for establishing quality standards of this valuable honey in Colombia.