The lethal concentration (CL) is used to estimate the toxicity of pesticides for arthropods. However, the CL is an incomplete measurement of the effects of products on populations because it analyzes only mortality as a parameter of toxicity. It is known that individuals that survive to exposure of pesticides may suffer sub-lethal effects. Objective: The lethal and sublethal effects of lime sulfur on the population growth of dust mites T. evansi and T. urticae were studied in laboratory conditions. Methodology: Lethal effects were estimated by using acute toxicity tests to obtain dosage-response curves and their respective lethal concentrations and sub-lethal effects were estimated by means of the instantaneous population growth rate (ri). Results: The lethal and sub-lethal concentrations of lime sulphur against T. evansi were 0.2 and 1.03%, respectively, whereas for T. urticae, concentrations were 5.68 and 23.44%. Conclusions: Lime sulfur represents a valuable alternative for T. evansi control; while for T. urticae is not a viable economically option to be recommended.