Was isolated and characterized a flocculant yeast strain Saccharomyces cerevisiae (LEG-06), to ferment banana juice, extracted under basic conditions. Yield ethanol was compared with commercial yeasts and were also evaluated the effects of pH and neutralizing agent. Additionally, the effect on efficiency of 10 successive fermentations using the same yeast. Finally, the fermentation was scaled in a 40 L tubular reactor with mechanical agitation and the kinetic parameters were determined from yeast. Ideal conditions were: pH 5,5 using citric acid as a neutralizer, 23,8 g/L yeast 14,5°Brix. The efficiency was between 82-84% and the optimum pH between 5,5 and 6,0 flocculation. The concentration of ethanol obtained under optimum conditions was 7,5%. These results demonstrate the yeast as the methodology used, have the right characteristics and desired to develop a process to pilot plant for the production of ethanol from banana rejection.