Introduction: The antimalarial drugs resistance from Plasmodium falciparum is related to point mutations in the essential proteins to the parasite biology. The K76T mutation in the Plasmodium falciparum Chloroquine resistance transporter (PFCRT) is a molecular marker resistance to Chloroquine. In Colombia the frequency of this mutation is 100%. Objective: To determine the frequency of K76T mutation in the Plasmodium falciparum CRT, infecting blood from people with malaria in the Colombia Caribbean zone with moderate transmission. Materials and methods: The DNA of the parasite was extracted from diagnostic slide using Chelex-100. A fragment of 148 pb of Pfcrt was amplified by PCR, the K76T mutation was evaluated by Restriction Fragment Length Polymorphism (RFLP) using endonuclease Apo I, the products were separated by polyacrylamide gel electrophoresis (PAGE). Results: Sixty six samples from people with malaria of six municipalities of Magdalena were studied. Fifty six DNA samples amplified of Pfcrt gene, T76 mutation was detected in 55 (98.2%). Occurrence of one sample carried the wild allele K76 would indicated the reversion of K76T mutation in Colombia, this phenomenon has been observed in some countries of the African continent, where recovery of sensitivity to Chloroquine was reported. Conclusions: This study is the first approach towards the knowledge of the antimalarials resistance in zones with moderate transmission in Colombia.