The increasing prevalence of allergic diseases in Colombia makes necessary to research about factors that influence their etiology. The aim of this study was to investigate the mite fauna present in homes of allergic child from Santa Marta. A total of 70 samples were collected from 42 houses through vacuuming mattresses and pillows, during the months of July to October 2007. Mites were identified under light microscopy using taxonomic keys, count and expressed as mites per gram of dust. The prevalent mite family was Pyroglyphidae (84.58%), having Dermatophagoides farinae as the major specie (23.85%), followed by D. pteronyssinus. Other mites prevalent were Cheyletus spp, Euroglyphus maynei and Blomia tropicalis, with levels up to 500 mites/ gram, considered like of high risk for the allergic sensitization. These results improve the knowledge of house dust mite fauna in Santa Marta, important by the diagnosis and therapy of allergies..