The biofilm is associated to water distribution system. It generates serious problems in the water quality and users complaints. This job presents a review of physical processes that origin biofilm and its control by flushing. Simulating different sceneries in pipe networks can define what zones have a major formation rate of biofilm than others. The parameters had into account are: pressure, flow velocity and permanency time of zone on critical conditions. The simulating can help to define if pressure and flow velocity are in an operative range according to policy authorities and have hydraulic operation criteria in a pipe network to control the biofilm growth.