This article reviews the main techniques for detecting apoptosis and cell senescence levels for application in animal and human cell cultures, given the importance of establishing the most appropriate methodology for implementing them in biological and biomedical research which uses these kinds of cell for diagnosis, research and in therapeutic alternatives. There is a need for implementing standardised techniques for assessing and monitoring cell cultures as this guarantees culture quality if they can be applied in the clinical field. Apoptosis and cell senescence appear to be good biological parameters for such evaluation. Apoptosis is considered to be a form of organised and programmed cell death, unlike necrosis; this process is energy