Este trabajo muestra los resultados experimentales de una serie de procesos biologicos unitarios para la remocion conjunta de materia organica (MO), nitrogeno (N) y fosforo (P), empleando un sustrato sintetico similar al agua residual domestica de concentracion media. Se utilizo un sistema tipo A2O (anaerobio-anoxico-aerobio), que se desarrollo en dos etapas. La etapa preliminar duro 60 dias y comprendio, ademas de la aclimatacion del lodo biologico, el ensayo de espuma de poliuretano, como material de soporte en la etapa aerobia. La etapa secundaria duro 280 dias mas, en los que el sistema alcanzo una estabilidad, lograndose un aumento notable en las eficiencias promedio de remocion de 92,5 % en materia organica, 87,7 % en nitrogeno y 83,5 % en fosforo. Se realizo un seguimiento permanente a parametros tales como el oxigeno disuelto (OD) y el potencial de oxido reduccion (ORP), que variaron de acuerdo con el proceso correspondiente a cada unidad de tratamiento, y el pH, que permanecio en valores superiores a 7,2 unidades, garantizando la viabilidad del proceso de nitrificacion desnitrificacion. Adicionalmente, se realizo un estudio hidraulico con trazadores para evaluar el efecto del material de soporte en el regimen hidraulico de la unidad aerobia. Abstract: experimental results from a series of biological unitary processes for the simultaneous removal of organic matter (OM), nitrogen (N) and phosphorus (P) are shown in this study. A synthetic substrate similar to a domestic wastewater of average concentration was used. The experimental development was made using an A2O system (anoxic-aerobic-anaerobic) performed in two phases. The preliminary stage lasted 60 days and included, besides the acclimatization of the biological sludge, the examination of polyurethane foam as a support medium in the aerobic phase. In the secondary stage, the stability was rapidly reached; this phase lasted 280 more days; a significant increase of the removal efficiencies was observed to be close to 92.5 % in organic matter, 87.7 % in nitrogen, and 83.5 % in phosphorus. Parameters such as dissolved oxygen (DO) and oxide reduction potential (ORP) were permanently monitored and maintained in values according to the requirements of the processes in each reactor. On the other hand, pH was measured to be higher than 7.2 units, ensuring the viability of the nitrification-denitrification processes. A hydraulic study using tracers was developed in order to find out the effect of the support medium on the hydraulic regimen of the aerobic reactor.