There are many important geographical areas in Colombia with a high agricultural potential. They have environmental constrains, however, to be used in certain periods during the year because of the low temperature. This happens in the Cundiboyasence plateau, in which, this phenomenon affects dramatically the growing of potato crops up to the point of causing a decrease in production or total loss. The purpose of this trial was to determine the presence of ABA in the following varieties of Solanum sp.: 'Capiro', 'Unica', 'Chitaga', 'Morita', 'Pastusa', 'ICA-Zipa' and 'Nativa'. The analysis was carried out in the pre-flowering period under an average environmental temperature of 20°C and exposed to simulated freezing conditions of -4°C. For the ABA extraction, leaves were collected, and subjected to liquid-liquid extraction and to thin layer chromatography technique. For the quantification, the length inhibition of wheat coleoptile (Triticum tensa vilgare L.) bioassay was used. In the study there were two kinds of response: susceptible and tolerant varieties. The varieties 'ICA-Zipa' and 'Chitaga' form the first group in which tolerance to frost stress was not observed. The varieties of the second group: 'Capiro ', 'Pastusa', 'Nativa', 'Unica' and 'Morita' showed toIerance to low temperature.