Cassava (Manihot esculenta Crantz) is a perennial shrub cultivated in Africa, Latin America and Southeast Asia. It is an important dietary source for humans in tropical countries. Carotenoids are natural pigments that are widely distributed in the nature, where about 50 of them have provitamin A activity, 4 ; ; ; ; ; evaluation of genetic diversity produced a dendrogram with six groups of 68% similarity. The average observed heterozygosity was Ht = 0.559. Regression and correlation analysis between content of total carotenoids and microsatellites markers showed that they were highly correlated with high levels of carotenoids and were found in linkage of the group D of the molecular and genetic cassava map.