It was carried out phytochemistry screening to twelve species with medicinal use, four Rubiaceae (Sabicea panamensis, Coccocypselum lanceolatum, Psychotria poeppigiana and Psychotria cooperi), and eight Gesneriaceae (Columnea consanguinea, Columnea picta, Columnea cruenta, Columnea parviflora, Drymonia killipii, Paradrymonia conferta, Paradrymonia darienensis and Besleria barclayi), were collected in four municipalities of the department of Choco (Colombia). A portion of 200g of leaves was marinated in cold with 96% ethanol. Extractions were concentrated successively in a reduced pressure rotavapor, following the described by Wall and his collaborators adapted with the methodology utilized by Sanabria. In the results of the phytochesmistry analysis, the alkaloids were presents in 3 of four species of the Rubiaceae with positive false results in 2 species of Gesneriaceae (Drymonia killipii y Besleria barclayi); the flavonoides were presents in all the Rubiaceae, while in Gesneriaceae alone it registered, their occurrence in 4 of them. The steroids were the constituent more common of the all the species, appearing in all the Rubiaceae and in most Gesneriaceae, the group of metabolites less common were nafto and/or antraquinonas, presents in abundance in Columnea cruenta and less quantity in Psychotria cooperi