The family Neritidae has representatives in tropical and subtropical regions that occur in a variety of environments, and its known fossil record dates back to the late Cretaceous. However there have been few studies of molecular phylogeny in this family. We performed a phylogenetic reconstruction of the family Neritidae using the COI (722 bp) and the 16S rRNA (559 bp) regions of the mitochondrial genome. Neighbor-joining, maximum parsimony and Bayesian inference were performed. The best phylogenetic reconstruction was obtained using the COI region, and we consider it an appropriate marker for phylogenetic studies within the group. Consensus analysis (COI +16S rRNA) generally obtained the same tree topologies and confirmed that the genus Nerita is monophyletic. The consensus analysis using parsimony recovered a monophyletic group consisting of the genera Neritina, Septaria, Theodoxus, Puperita, and Clithon, while in the Bayesian analyses Theodoxus is separated from the other genera. The phylogenetic status of the species from the genus Nerita from the Colombian Caribbean generated in this study was consistent with that reported for the genus in previous studies. In the resulting consensus tree obtained using maximum parsimony, we included information on habitat type for each species, to map the evolution by habitat. Species of the family Neritidae possibly have their origin in marine environments, which is consistent with conclusions from previous reports based on anatomical studies. Filogenia molecular de la familia Neritidae (Gastropoda: Neritimorpha) con base en los genes mitocondriales citocromo oxidasa I (COI) y 16S rRNA La familia Neritidae cuenta con representantes en regiones tropicales y subtropicales adaptadas a diferentes ambientes, con un registro fosil que data para finales del Cretaceo. Sin embargo no se han realizado estudios de filogenia molecular en la familia. En este estudio se realizo una reconstruccion filogenetica de la familia Neritidae utilizando las regiones COI (722 pb) y 16S rRNA (559 pb) del genoma mitocondrial. Se realizaron analisis de distancias de Neighbor-Joining, Maxima Parsimonia e Inferencia Bayesiana. La mejor reconstruccion filogenetica fue mediante la region COI, considerandola un marcador apropiado para realizar estudios filogeneticos dentro del grupo. El consenso de las relaciones filogeneticas (COI+16S rRNA) permitio confirmar que el genero Nerita es monofiletico. El consenso del analisis de parsimonia revelo un grupo monofiletico formado por los generos Neritina, Septaria, Theodoxus, Puperita y Clithon, mientras que en el analisis bayesiano Theodoxus se encuentra separado de los otros generos. El resultado en las especies del genero Nerita del Caribe colombiano fue consistente con lo reportado para el genero en estudios previos. En el arbol resultante del analisis de parsimonia se sobrepuso la