The hantaviruses associated to the Sigmodontinae rodents, are emerging pathogens that cause the Cardiopulmonary Syndrome by Hantavirus (HCS) in the Americas. So far, Colombia has not reported confirmed cases of the disease; however, evidence has shown serological exposure to hantavirusin rodents and humans from two departments in the Caribbean Region, and molecular detection of viral genome in tissue samples of rodents in Antioquia. The aim of this research was to determinate the frequency of hantavirus specific antibodies in rodents from Sincelejo City. Animal samplings were performed with Sherman traps from March to July 2009. IgG antibodies against Sin Nombre Virus were detected in plasma by enzyme-linked immunosorbent assay(indirect ELISA). Sixty-one rodents were captured and identified as three speciesthree species, Musmusculus was the most abundant(91.8%). From the total of analyzed animals, only one exemplar, belonging to Rattusrattus specie (seroprevalence 1.64%), was positive to hantavirus. Serologic findings evidenced hantavirus infection among rodents of subfamily Murinae in Colombia. The presence of antibodies against the virus suggests that at least one hantavirus is circulating in rodents from North of Colombia.