This writing shows the design of a preventive maintenance protocol that covers a complete routine of preventive maintenance, washing, disinfection and predictive maintenance in all components of a water processing area used in hemodialysis machines, preserving the safety of the personnel in charge and the health of the patient. The usefulness of the protocol in different institutions is evaluated by means of a survey and it was shown that the designed protocol would be very useful in the efficacy and safety in the water treatment processes used in hemodialysis, in different institutions that perform this procedure in renal units or intensive care units. Through its application, procedures would be standardized, personnel would be trained with centralized information, and quality processes in water treatment would be guaranteed.