Introduction: The diagnosis and treatment of early neonatal sepsis (NTS) in Colombia are based by the Clinical Practice Guide of the Ministry of Health and Social Protection published in 2013, a document that discourages the use of scales or other tools in the approach of this pathology. However, recent world literature supports the use of the neonatal sepsis calculator as a useful and safe tool for identifying the risk of NTS. Objective: To determine the concordance between the clinical recommendations obtained from the Colombian clinical practice guideline for the management of (early) neonatal sepsis, and those obtained from the implementation of an early neonatal sepsis calculator, in three third-level hospitals in Bogotá. Methodology: Multicenter, observational analytical concordance study with retrospective collection. Infants who were born between January 2017 and December 2019, ≥34 weeks' gestation, and admitted to the neonatal unit for suspected early neonatal sepsis were included. Both the incidence of TNS and the proportion of infants recommended for antibiotics by Colombian CPG versus the theoretical application of the neonatal sepsis calculator were evaluated. Results: Four hundred and seventy (1.71%) of the twenty-three thousand four hundred and ninety-seven births attended over 34 weeks were admitted and managed in the postnatal care room due to suspected early neonatal sepsis. 41.7% were female, with a median gestation of 37.14 weeks (IQR: 3.43 weeks) and median birth weight of 2700 g (IQR: 716 g). Seven patients were confirmed by blood cultures with early neonatal sepsis, with group B Streptococcus being the most frequent microorganism (57%; 95% CI: 18.40-90.10). One death occurred in the group of patients with neonatal sepsis, which is equivalent to a case fatality rate of 14.3%. The global incidence of early neonatal sepsis was estimated at 0.233 per 1000 NB. When dichotomizing the recommendations following 2 different scenarios, unequivocal and cautious, the level of agreement between the calculator and the CPG did not exceed 20% (6% vs. 14%, respectively), that is, poor agreement. Conclusions: The CPG presents a low concordance with the Kaiser Permanente calculator, where it had a better predictive capacity when detecting all newborns with neonatal sepsis. Although the use of the calculator could bring clinical and administrative advantages, more prospective studies on the safety of the calculator implementation are warranted, taking into account the practices between the different health care services in the Colombian context.