Colombia has a great potential to produce biofuels based on biomass residues due to the importance of agroindustry in the country's economy. The diversity of this industry guarantees the availability of different residues which are susceptible to be valorised through biological processes. There are several works, in the country, focused on biomass use to produce bioethanol and biodiesel. Nevertheless, the biohydrogen production using residual biomass has been scarcely studied. Dark fermentation and anaerobic digestion