During the last decade, biodiesel has emerged as a promising alternative to diesel. However, the sedimentation of some insoluble material in pure biodiesel has become a concern for its producers. This material easily drops out of solution, settles on the bottom of biodiesel storage tanks and forms deposits on vehicle fuel filters. In this work, the insoluble material that forms in Colombian palm oil biodiesel was characterized for the first time. Non-expensive and easy to use Thin Layer Chromatography and Fourier Transform Infrared