Competing between foods and biofuels production, the world food crisis and high oil prices have ignite attention in algaculture for making biofuels. Among algae biodiesel positive characteristics are: it can be produced using sea water and wastewater without affecting fresh water resources, is biodegradable and relatively harmless to the environment if spilled. This work represents the first attempt of optimizing thirdgeneration biodiesel production by homogeneous transesterification. It was used a synthetic algae oil based on Chorella