Myzus persicae is considered the third pest of economic importance in Chile affecting several crops. Its genetic variability is influenced by the availability of the primary host. M. persicae has acquired resistance to most of the insecticides used for its control. The current work aimed to evaluate the genetic diversity and the presence of resistance mechanisms of M. persicae in crops, such as peach and sweet pepper. The study was carried out in twelve localities, six in the O'Higgins region and six in the Maule region of central Chile. Monthly