In this study, the quantification of scopolamine from Brugmansia candida normal roots is described. The roots were obtained and cultured in half salts strength Schenk and Hildebrandt (1/2 SH) liquid medium plus 30 gl− 1 sucrose and supplemented with 3 mg l− 1 of α-naphthaleneacetic acid. The quantitative determination of scopolamine was carried out by HPLC. The maximum scopolamine production in Brugmansia candida normal root culture was 3.94±0.01 mg g− 1 dry weight obtained at 24th day of culture.
