DNA variation was studied at the intra-andinterspecific levels within the genus Passiflora, with particular emphasis on the subgenus Tacsonia and its relationship with the subgenus Passiflora. AFLP fingerprints were generated for35 accessions from 21 species, 14 of which belong to subgenus Tacsonia, and four to subgenus Passiflora. The morphologically intermediate P. manicata (subgenus Manicata) was also included, as were P. trinervia (subgenus Psylanthus) and P. alnifolia (subgenus Decaloba). The two latter were used as