Molecular data were used to evaluate the validity of the genus Neopisosoma Haig, 1960. Comparisons of morphological features within Neopisosoma suggest the existence of two lineages, represented among others, by N. angustifrons (Benedict, 1901) and N. neglectum Werding (1986). Both lineages of Neopisosoma are more similar to two morphologically different species groups of the genus Pachycheles, than to congeners of the other group. Comparative morphology of larvae from N. angustifrons, N. neglectum and species of