This study aimed to produce and characterise laminar biochar from Guadua angustifolia Kunth culms longitudinal cuts by TiO 2 sedimentation deposit with photocatalytic activity against bacteria; several ages and height positions culms served to produce longitudinal cuts, fibre and parenchyma compositions; longitudinal cuts containing the highest fibre were transformed into laminar biochar by modified pyrolysis; biochar TiO 2 deposited served to evaluate the photocatalytic activity against Escherichia coli 226 and Salmonella Typhimurium 211, the highest fibre percentages for longitudinal cuts were MM, BM, TJ, TD, and DM (ranging from 59.50 to 63.78 %), among these, BM, MD and TD showed the highest thermal stability, with decomposition temperatures between 341.65 and 345.85 ºC. Elastic modulus in bending (E) for these cuts exceeded 3.06 GPa, indicating their mechanical strength. TiO 2 deposited on the laminar biochar exposed to UV 253nm generated > 50 % inactivation of both bacteria after 30 min of exposure (photocatalytic activity).