This study examines occupational exposure to perchloroethylene among dry cleaning workers in Bogotá, Colombia, where its use remains prevalent despite global trends toward alternatives. A cross-sectional analysis was conducted with 9 workers to measure perchloroethylene exposure levels and blood concentrations. Additionally, a case-control study assessed gamma glutamyl transferase (GGT) and alanine aminotransferase (ALT) as biomarkers for hepatocellular damage. Workers were classified into 3 job categories: dry cleaning operators, ironers, and cold washing operators. Results indicated that dry cleaning operators experienced significantly higher exposure levels (0.72 to 7.22 ppm) compared to ironers and cold washers (0.001 to 3.39 ppm). All exposure levels were below the adjusted threshold limit of 19.58 ppm, and blood perchloroethylene concentrations were below detection limits. No significant differences in GGT and ALT levels were observed between exposed workers and the control group. Given the small sample size, further research is warranted to better understand exposure risks in this sector. Emphasizing a culture of prevention is crucial due to the carcinogenic potential of perchloroethylene, even at low environmental exposure levels.