Multifocal glioblastoma represents a therapeutic challenge with conflicting evidence on treatment, though it appears that the extent of resection also plays an important role in this disease. The use of 5-aminolevulinic acid has been shown to improve the extent of resection in high-grade gliomas; however, there is little information about its use in multifocal glioblastoma. We present the case of a 79-year-old patient with distant glial lesions who underwent surgery for the resection of a right temporal and a right occipital lesion at the same surgical time. Intraoperative findings and postsurgical events are described in this report. This case shows that resection of multiple lesions in a patient with multifocal glioblastoma using intraoperative 5-aminolevulinic acid fluorescence is feasible and safe.