ABSTRACT Quinoa is a pseudocereal of great importance because of its nutritional content, great adaptability and broad genetic variability. In Colombia, genetic studies on quinoa are scarce, and there are no certified varieties. Rather, varietal mixtures that affect grain quality and, therefore, postharvest and marketing processes grow in the field. The objective of this study was to carry out a morphoagronomic characterization of five quinoa cultivars under field and greenhouse conditions in Tunja-Boyacá using 21 qualitative and quantitative descriptors, estimating their variation with descriptive, multivariate, and cluster analyses. The results show a high phenotypic segregation among cultivars, where yield, diameter, and panicle length were the quantitative variables with greater variation both in the field and in the greenhouse. The multivariate analysis showed that 72.54% of the variance observed in the greenhouse and 75.20% in the field were explained by the first two components, where plant height, stem diameter, panicle density and color, and stem color were discriminating variables. In the field and in the greenhouse, three groups of cultivars were formed according to the yield and desirable morphoagronomic characteristics. Finally, under the present environmental conditions, the more promising quinoa materials for a breeding program were Blanca Subachoque and Tunkahuan.