Queen conch (Aliger gigas) pearls has been appreciated since pre-Columbian time and exported for years due to their qualities which have made them suitable for jewellery. In this study was obtained characteristics of the natural pearls, information of the specimens that produced them as well as the probability of finding a pearl from industrial (San Andrés Archipelago -SAA) and artisanal fisheries (San Bernardo Archipelago-SBA) in Colombia. In order to approach a technique to culture conch pearls, it was carried out two assays in San Martin de Pajarales Island (SBA) to assess relaxants, implantations, maturity, presence of flared shell lip (sub-adults and adults), sex, nuclei material and nuclei size. Three nuclei material were used: small-conch-pearls, polish-conch-shell and resin. There were a total of 20 natural pearls found in fisheries. Their weight ranged from 0.05 to 25.95 Ct —carat— and 50% of them presented a flame structure; 75% of them exhibited a rounded shape and 70% a red colour. The probabilities of finding a pearl in SAA (1:1025) and SBA (1: 961) were similar to other areas of the Caribbean. It was found that most of these pearls were produced in the inner lip of the collar of mantle (94%) by sub-adults and adults (90.9%). According to the assays, the relaxant magnesium chloride was 70% effective at 2.5 g L-1 after 30 minutes and suitable for implantations. Specimen's survival was 100% after nuclei implantation and 94.8% after extraction. Nuclei material, nuclei size, maturity and sex did not show an effect on nuclei rejection. Specimens with flared shell lip were more suitable for the implantations. Implantations were successful since we obtained more than 70% of nucleus retention at 30 days post-implantation. We obtained a polished-conch-shell nucleus recovered with a calcium carbonate layer of 0.3 mm thick. This «cultured pearl» had a size of 4.67x4.32x3.62 mm and growth rate of 0.0039 mm·day-1. This study discussed the technique to culture conch pearls and guide further studies. Finally, we make an appeal to protect this valuable species in the Caribbean.