Organochlorine insecticides and polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) that accumulate in the food chain, persist in the environment, and pose health risks, even in small quantities from contaminated food, such as tropical fruits. This study assessed the levels of these pollutants in tropical fruits and soils from Antioquia, Colombia, and evaluated associated health risks from consumption. A total of 56 fruit samples (Hass avocado, cape gooseberry, and purple passion fruit) and 32 soil samples were collected from eight farms between March 2023 and January 2024. Samples were analyzed using gas chromatography coupled with mass spectrometry. Results revealed trace amounts of heptachlor and gamma-chlordane, but below the regulatory limit of 10 µg/kg. The concentration of 4,4'-DDT was higher than 4,4'-DDE in soil, with ratios greater than one (1.11–6.12), indicating industrial dicofol contamination. No PCBs were detected in fruit samples, but consistent levels were observed in soil. No carcinogenic or non-carcinogenic health risks were identified in the fruit samples, though non-carcinogenic risks were observed. The study emphasizes the need for ongoing monitoring to reduce exposure risks for the local population and highlights the importance of further research to inform environmental health policies.