This study examined the ectoparasites in free-living wild bee colonies in Totoró, Cauca Department, and Valle del Cauca, Colombia. Researchers collected 2116 bee specimens: 620 from Cauca (200 Apis mellifera , 320 Partamona peckolti , and 100 Paratrigona eutaeniata ) and 1496 from Valle del Cauca (Dagua, Tocota, Buga, Cali, Pradera), including 1498 A . mellifera and 48 Tetragonisca angustula . Using microscopic and stereoscopic techniques and a special taxonomic key, the study identified the first recorded presence of Leptus (Leptus) alberti n. sp. (Acari: Erythraeidae) in colonies of free-living Africanized A . mellifera and stingless bees, including P . peckolti , P . eutaeniata , and T . angustula in Cauca and Valle del Cauca. This finding updates the reported species for South America. The presence of L . (L . ) alberti n. sp. was identified in four sample points in the Totoró-Cauca area, with a 75% parasitic prevalence in free-living bee colonies at three of the four localities, specifically in hives located in the wild forest of Totoró. Two Meliponini species were also identified: one in P . peckolti and another in P . eutaeniata . In Cauca, the prevalence was 5% for A . mellifera and 0.3% for Meliponini. In Valle del Cauca, L . alberti n. sp. was observed in five out of 22 localities, resulting in a 23% prevalence in wild A . mellifera . Additionally, a 4.16% prevalence (2/48) of L . alberti n. sp. was found in T . angustula . Six A . mellifera specimens tested positive for L . (L . ) alberti n. sp., as did one P . peckolti and one P . eutaeniata specimen. No other external mites were detected in the collected samples.