Introduction: Human papillomavirus (HPV) is one of the most important oncogenic viru-ses detected in different types of cancer, which includes esophageal squamous cell carcino-ma (ESCC) and gastric cancer (GC). However, Colombia does not have data on the associa-tion of HPV with GC. Objetive: The aim of this study was to determine the presence HPV genotypes in patients with ESCC and GC, diagnosed between 2007 and 2014 at the Hospital Central de la Policía Nacional of Bogotá-Colombia (HOCEN).Methods: A total of 33 paraffin embedded tissue samples were examinate by polymerase chain reaction for the L1 gene of HPV. The extracted DNA was amplified using the universal primers MY09 and MY11. Then, the positive samples had high risk HPV genotyping perfor-med on them. The samples were analyzed by Roche Cobas 4800 system.Results: Five (15%) samples identified as positive to HPVDNA [four men (80%) and one woman (20%)], one (20%) was positive for HPV-16. The other four, whose genotypes had not detected by COBAS 4800, samples could correspond to low-risk HPV, or match to some of the high-risk genotypes that had not included in the detection panel of this test. Also, four (80%) HPVDNA positives had identified as adenocarcinoma intestinal type, and one (20%) as ESCC. Conclusion: Our study proves the existance of HPV and p16 in Colombian patients with ESCC and GC, that could be a risk factor for the development of these pathologies in this population.