There is great concern in the field of public health and sports ethics about how doping and gene doping are transforming athletes. This systematic review aims to analyze the current state of research on the contribution of biotechnology to sport and how this affects the health of athletes, bodybuilders, and high-intensity exercisers. The search includes articles published between 2014 and 2024; for this, databases without language restriction were used, finding 93 articles in the first search; finally, a total of 8 studies were selected in terms of the methodological process through the variables: biotechnology, sport, doping, and health. Studies on biotechnology and sports show that athletes continue to use substances to increase physical performance, and bodybuilders use them to improve size and aesthetic appearance.