Abstract The penis is a structure that requires both anatomical and functional reconstruction. Being a three-dimensional structure that changes in volume, it presents a reconstructive challenge for the plastic surgeon. Currently, various alternatives are available for covering these complex defects, such as grafts, flaps, and dermal matrices. The objective of this study is to present a case report describing a complex post-traumatic penile defect in which three combined strategies were implemented for the first time (spiral full-thickness grafts, nanolipoinjection, and negative pressure therapy). These strategies have been described in the literature for defect coverage, achieving satisfactory anatomical and functional results.