Fire plays a crucial role in shaping multiple ecological processes in native savannas. Still, the natural fire regime in the Neotropical savanna of South America is being altered by climate variability, resulting in reduced precipitation and increased mean temperatures. In recent years, an increase in the duration, intensity, frequency, and extent of wildfires in South American savannas has been reported. However, knowledge of the ecology of wildfires in the savanna landscapes and their impact on the habitats of large mammals is limited or non-existent. This study investigates the impact of wildfires on the suitability habitat for Tapirus terrestris in a native high plains native savanna landscape in the department of Vichada in eastern Colombia. We used the MaxEnt algorithm to model the ecological niche of the lowland tapir and then classify land covers into low, medium, and high probability of species occurrence. We then assessed the representativeness of the occurrence of wildfires and the frequency of detection of burned areas within the land covers classified into the suitability model of lowland tapir. Our results indicate that wildfires significantly affect high-suitability habitats such as forest and shrubland covers. The effects of fire on these land cover types would reduce the resource supply for lowland tapir and cause the displacement of individuals to new areas which could increase the exposure to threats such as hunting and pathogens from domestic animals. This research underscores the need for targeted conservation strategies to mitigate wildfire impacts and preserve critical habitats for vulnerable species.