The neurological rehabilitation focuses on enhancing functional recovery and improving the quality of life for people who have experienced injuries or diseases affecting the central or peripheral nervous system. This functional recovery includes a follow up of the kinematics of the patients limbs. The use of a open source software such as OpenSim, has been previously proposed as a tool for kinematic analysis, this software allows for highly specialized 3D musculoskeletal modeling, facilitates kinematic analysis and the assessment of force and angles in the lower and upper limbs of the human body. In this context, the propose of this research was to test the reliability of OpenSim for kinematic analysis during neurological rehabilitation. For this goal, the Motricity Index test was done by a group of three healthy participants, this values were used for comparison to the stroke patient who is currently undergoing neurological rehabilitation process. The results demonstrates all the limitations in the range of motion of the patient in comparison to the healthy group due his motor issues, such as muscle spasticity and weakness. This research shows the advantages and limitations of this software and its application in neurological rehabilitation. The goal is to contribute to the development of effective and personalized therapeutic strategies to improve the recovery process for these patients.