Vector-borne pathogens induce hemoparasitism in cattle causing substantial economic losses in tropical and subtropical areas. Infectious cattle actively contribute to maintaining the transmission cycle, and the presence of these animals must be associated with husbandry management and environmental changes. In the present study, we conducted a cross-sectional study sampling 1,000 bovines to identify infectious cattle diagnosed by a direct technique and employed a dichotomic questionnaire for association analyses, hierarchical clustering, and Principal Component Analysis (PCA). Overall prevalence with infectious cattle was 34.99%, where 97% of the farms had at least one infectious animal per genera, and the prevalence in properties ranged between 16.39 and 53.85%. Of these animals, 26.20% tested positive for